Adiponectin and colon cancer: evidence for inhibitory effects on viability and migration of human colorectal cell lines


Adiponectin (Acrp30) is an adipokine widely studied for its beneficial metabolic and anti-inflammatory properties. Colorectal cancer is among the most common cancers worldwide. The aim of present study was to explore the effects of Acrp30 on both CaCo-2 and HCT116 colorectal cancer cells in terms of viability, oxidative stress, and apoptosis. In addition, since colorectal cancer represents a typical inflammation-related cancer, we investigated whether Acrp30 treatment modifies the migration and the expression of crucial proteins in the EMT transition. Finally, we analyzed the expression of cytokines in CaCo-2 cells. We found that Acrp30 reduces the survival rate of both CaCo-2 and HCT116 cells through induction of apoptosis and oxidative stress already after 24 h of treatment. In addition, wound-healing assay indicated that Acrp30 exposure statistically inhibits CaCo-2 and HCT116 cell migration. Western blot analysis performed on E-cadherin and vimentin, two EMT crucial markers in carcinogenesis, indicated that Acrp30 does not influence EMT transition. Finally, we found a reduction of mRNA levels corresponding to the anti-inflammatory IL-10 cytokine together with an increase of the pro-inflammatory IL-6 and IL-8 cytokines. This study provides new insight into Acrp30 molecular effects on colorectal cancer cells. Indeed, even if further studies are necessary to clarify the precise role of Acrp30 in colorectal cancer, our data strongly suggest that Acrp30 negatively regulates cell survival and migration in association with induction of oxidative stress and regulation of cytokines expression in both CaCo-2 and HCT116 colorectal cells.


Adiponectin; Cell proliferation and apoptosis; Colorectal cancer; Cytokines; Migration



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Giornata mondiale per la lotta al cancro del colon-retto. ” La Prevenzione Salva la Vita “


Giornata mondiale per la lotta al cancro del colon-retto. ” La Prevenzione Salva la Vita ” #pietroschettino Cancer Day is an international day marked on 4th February all over the world to raise awareness of cancer and to encourage its prevention, detection, and treatment. World Cancer Day was founded by the Union for International Cancer Control (UICC) to support the goals of the World Cancer Declaration, written in 2008. The primary goal of the World Cancer Day is to significantly reduce illness and death caused by cancer by 2020. The theme for the 2017 is “We can. I can”. #surgicalendoscopy #endoscopy #prevention #life #ccr #colon #colón #coloncancertest #health #medicine #chirurgia #endoscopia #chirurgiaendoscopica #coloncancer #coloncanceralliance #coloncancerscreening #screening #gastroenterologia #colonscopia #italia #italy

Una foto pubblicata da Dott. Pietro Schettino (@surgicalendoscopy) in data: 4 Feb 2017 alle ore 08:45 PST

Congresso Congiunto delle Società Scientifiche Italiane di Chirurgia – Roma 2016

Congresso Congiunto delle Società Scientifiche Italiane di Chirurgia #chirurgiaunita2016 #surgicalendoscopy #surgery…

Pubblicato da I.S.S.E. ITALIAN SOCIETY FOR SURGICAL ENDOSCOPY su Venerdì 30 settembre 2016Una foto pubblicata da Dott. Pietro Schettino (@surgicalendoscopy) in 14440670_10209301219040858_7397876667296514707_n

data: 29 Set 2016 alle ore 21:37 PD


#pietroschettino @surgicalendoscopy



Congresso Internazionale

“la Casa della speranza”

Prevenzione diagnosi e cura
della patologia neoplastica in “Campania Felix”

Convegno Primaverile ISSE

Hot topics in chirurgia endoscopica

12 | 13 giugno 2015

Aula magna Scuola Superiore della Pubblica Amministrazione Viale Douhet, 2/A Palazzo Reale di Caserta

A. Pezzullo F. Fiocca V. Menniti

CoordInatorI Scientifici:

G. D. de Palma , P. Schettino